Construction strategies for developing expression vectors for recombinant monoclonal antibody production in cho cells authors authors and affiliations 2008 assessment of cell engineering strategies for improved therapeutic protein productioon in cho cells evaluating the efficiency of chef and cmv promoter with ires and furin 2a . Today mammalian cell lines represent the most widely used expression system for the production of recombinant antibodies several other hosts are being developed which are even able to produce antibodies with human like glycosylation patterns. Antibody expression and production cell engineering engineered antibodies currently represent over 30 of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly medical books antibody expression and production . The biotechnology and applications of antibody engineering the use ofin vitro immunization cloning of variable regions and scid mice for the production of human monoclonal antibodies in voss e w and jacobs t w 1991 single chain antibody sca encoding genes one step construction and expression in eukaryotic cells
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